DCC Recombinant Rabbit Monoclonal Antibody [PSH12-73]
cat.: HA723484
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH12-73 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 158 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DCC aa 1-1,097. |
| Positive control: | Mouse brain (P0) tissue lysate, Mouse brain (P7) tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P43146 Human | P70211 Mouse | Q63155 Rat |
| Alternative names: | Colorectal cancer related chromosome sequence 18 Colorectal cancer suppressor CRC 18 CRC18 CRCR 1 CRCR1 DCC DCC_HUMAN Deleted in colorectal cancer protein Deleted in colorectal carcinoma Immunoglobulin superfamily DCC subclass member 1 Netrin receptor DCC Tumor suppressor protein DCC |
Images
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Fig1:
Western blot analysis of DCC on different lysates with Rabbit anti-DCC antibody (HA723484) at 1/5,000 dilution. Lane 1: Mouse brain (P0) tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Mouse brain (P7) tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 158 kDa Observed band size: 200 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723484) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: E14.5 embryo (brain) Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.