PHD2 Recombinant Rabbit Monoclonal Antibody [PSH12-74]
cat.: HA723485
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH12-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PHD2 aa 151-426. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, HCT 116 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9GZT9 Human |
| Alternative names: | C1ORF12 Chromosome 1 Open Reading Frame 12 DKFZp761F179 ECYT 3 ECYT3 Egl 9 family hypoxia inducible factor 1 EGL 9 homolog of C. elegans 1 EGL nine (C.elegans) homolog 1 Egl nine homolog 1 (C. elegans) Egl nine homolog 1 Egl nine like protein 1 EGLN 1 Egln1 EGLN1_HUMAN HIF PH2 HIF Prolyl Hydroxylase 2 HIF-PH2 HIF-prolyl hydroxylase 2 HIFP4H 2 HIFPH2 HPH 2 HPH-2 HPH2 Hypoxia inducible factor prolyl hydroxylase 2 Hypoxia-inducible factor prolyl hydroxylase 2 ORF13 P4H2 PHD 2 PhD2 PNAS 118 PNAS 137 Prolyl Hydroxylase Domain Containing Protein 2 Prolyl hydroxylase domain-containing protein 2 Rat Homolog of SM20 SM 20 SM-20 SM20 Zinc finger MYND domain containing protein 6 ZMYND6 |
Images
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Fig1:
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (HA723485) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723485) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (HA723485) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PHD2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723485) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.