LDHA + LDHB Recombinant Rabbit Monoclonal Antibody [PSH12-75]
cat.: HA723486
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH12-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LDHA aa 1-50 / 332. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, A431 cell lysate, 293T cell lysate, PANC-1 cell lysate, U-87 MG cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, human breast cancer tissue, human liver tissue, mouse liver tissue, mouse heart tissue, rat heart tissue, HeLa, C2C12, PC-12. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC IP |
1:5,000 1:20,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P07195 Human | P00338 Human | P16125 Mouse | P06151 Mouse | P42123 Rat | P04642 Rat |
| Alternative names: | Cell proliferation-inducing gene 19 protein GSD11 L lactate dehydrogenase B chain L-lactate dehydrogenase A chain Lactate dehydrogenase A Lactate dehydrogenase B Lactate dehydrogenase c variant 1 Lactate dehydrogenase c variant 3 Lactate dehydrogenase c variant 4 Lactate dehydrogenase C4 Lactate dehydrogenase H chain Lactate dehydrogenase M LDH A LDH B LDH H LDH heart subunit LDH M LDH muscle subunit LDH-A LDH-M LDH1 ldha LDHA_HUMAN LDHBD LDHM MS1111 PIG19 Proliferation inducing gene 19 Proliferation-inducing gene 19 Renal carcinoma antigen NY REN 46 Renal carcinoma antigen NY-REN-59 TRG 5 TRG5 |
Images
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Fig1:
Western blot analysis of LDHA + LDHB on different lysates with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: A431 cell lysate Lane 5: 293T cell lysate Lane 6: PANC-1 cell lysate Lane 7: U-87 MG cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: RAW264.7 cell lysate Lane 11: Neuro-2a cell lysate Lane 12: C2C12 cell lysate Lane 13: C6 cell lysate Lane 14: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723486) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723486) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723486) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723486) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723486) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723486) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunocytochemistry analysis of HeLa cells labeling LDHA + LDHB with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Immunocytochemistry analysis of C2C12 cells labeling LDHA + LDHB with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig9:
Immunocytochemistry analysis of PC-12 cells labeling LDHA + LDHB with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDHA + LDHB antibody (HA723486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig10:
Flow cytometric analysis of HeLa cells labeling LDHA + LDHB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723486, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig11:
Flow cytometric analysis of C2C12 cells labeling LDHA + LDHB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723486, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig12:
Flow cytometric analysis of PC-12 cells labeling LDHA + LDHB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723486, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig13:
LDHA + LDHB was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723486 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723486 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723486 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723486 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 9 seconds; ECL: K1801 |
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