Human TSLP Recombinant Rabbit Monoclonal Antibody [PSH12-90]
cat.: HA723491
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TSLP aa 29-159 (HA210952). |
| Positive control: | Recombinant Human TSLP protein (HA210952). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-89] to Human TSLP antibody (Capture) (HA723490) and recombinant Human TSLP protein (HA210952) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: Q969D9 Human |
| Alternative names: | Thymic stromal lymphopoietin Thymic stromal lymphopoietin protein TSLP Tslp TSLP protein TSLP_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human TSLP matched pair antibodies Capture: HA723490, Human TSLP Rabbit mAb [PSH12-89] Detector: HA723491, Human TSLP Rabbit mAb [PSH12-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723490) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked TSLP in human cell culture media samples. The concentrations of TSLPwere interpolated from the TSLP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 25%, cell culture media 2 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.