TMEM119 Recombinant Rabbit Monoclonal Antibody [PSH12-98]
cat.: HA723503
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH12-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse TMEM119 aa 113-280. |
| Positive control: | SH-SY5Y cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, SH-SY5Y. |
| Subcellular location: | Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:2,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q4V9L6 Human | Q8R138 Mouse Entrez Gene: 304581 Rat |
| Alternative names: | OBIF Osteoblast induction factor PSEC0199 Transmembrane protein 119 UNQ731/PRO1415 |
Images
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Fig1:
Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (HA723503) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 35/55 kDa Exposure time: Lane 1: 3 minutes; Lane 2-4: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723503) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling TMEM119 with Rabbit anti-TMEM119 antibody (HA723503) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TMEM119 antibody (HA723503) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling TMEM119. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723503, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
TMEM119 was immunoprecipitated from 0.2 mg mouse cerebellum tissue lysate with HA723503 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723503 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse cerebellum tissue lysate (input) Lane 2: HA723503 IP in mouse cerebellum tissue lysate Lane 3: Rabbit IgG instead of HA723503 in mouse cerebellum tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.