ULBP3 Recombinant Rabbit Monoclonal Antibody [PSH13-03]
cat.: HA723508
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH13-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ULBP3 aa 21-220. |
| Positive control: | MDA-MB-231 cell lysate, MCF7 cell lysate, SW480 cell lysate, HeLa cell lysate, human placenta tissue, human stomach tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q9BZM4 Human |
| Alternative names: | ALCAN- gamma ALCAN-gamma N2DL-3 N2DL3 N2DL3_HUMAN NKG2D ligand 3 NKG2D ligand 3 precursor NKG2DL3 OTTHUMP00000017411 RAET1N Retinoic acid early transcript 1N UL16 binding protein 3 UL16-binding protein 3 ULBP3 |
Images
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Fig1:
Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA723508) at 1/5,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (low expression) (20 µg/Lane) Lane 3: SW480 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723508) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ULBP3 antibody (HA723508) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723508) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ULBP3 antibody (HA723508) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723508) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
ULBP3 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723508 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723508 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723508 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723508 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 42 seconds; ECL: K1802 |
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Fig5:
Western blot analysis of ULBP3 on different lysates with Rabbit anti-ULBP3 antibody (HA723508) at 1/5,000 dilution. Lane 1: His-tagged Human ULBP3 recombinant protein Lane 2: His-tagged Human ULBP1 recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723508) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.