HRP Conjugated Mouse Factor XII Recombinant Rabbit Monoclonal Antibody [PSH12-22]
cat.: HA723509H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH12-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Factor XII aa 20-597 (HA210984). |
| Positive control: | Recombinant Mouse Factor XII protein (HA210984). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-21] to Mouse Factor XII antibody (Capture) (HA723440) and Recombinant Mouse Factor XII protein (HA210984) as the standard. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q80YC5 Mouse |
| Alternative names: | Factor XII Beta factor XIIa part 1 Beta factor XIIa part 2 Coagulation factor XII Coagulation factor XIIa heavy chain Coagulation factor XIIa light chain F12 F12 deficiency FA12_HUMAN Factor XII deficiency HAE3 HAEX HAF HAF deficiency Hageman factor |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723441, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.