CD11c Recombinant Rabbit Multiclonal Antibody [PSH13-25]
cat.: HA723512
| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-Fr |
| Clone number: | PSH13-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 129 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD11c aa 616-936 / 1,169. |
| Positive control: | THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate, RAW264.7 cell lysate, Mouse colon tissue lysate, Mouse spleen tissue lysate, mouse spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P20702 Human | Q9QXH4 Mouse |
| Alternative names: | 95 alpha chain 95 CD 11c CD11 antigen like family member C CD11 antigen-like family member C CD11c CD11c antigen Complement component 3 receptor 4 subunit CR4 Integrin alpha X Integrin alpha X chain Integrin alpha-X Integrin aX Integrin subunit alpha X integrin, alpha X (antigen CD11C (p150), alpha polypeptide) integrin, alpha X (complement component 3 receptor 4 subunit ITAX_HUMAN ITGAX LEU M5 alpha subunit Leu M5 Leukocyte adhesion glycoprotein p150 95 alpha chain Leukocyte adhesion glycoprotein p150 Leukocyte adhesion receptor p150 95 Leukocyte adhesion receptor p150 Leukocyte surface antigen p150 95 alpha subunit Leukocyte surface antigen p150 alpha subunit Myeloid membrane antigen alpha subunit p150 95 integrin alpha chain p150 p150/95 SLEB6 |
Images
|
Fig1:
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA723512) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723512) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.