Human CCL21 Recombinant Rabbit Monoclonal Antibody [PSH13-36]
cat.: HA723518
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL21 aa 24-134 (HA211144). |
| Positive control: | Recombinant Human CCL21 protein (HA211144). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-37] to Human CCL21 antibody (Detector) (HA723519) and recombinant Human CCL21 protein (HA211144) as the standard. The reference range value is 8.2-2,000 pg/mL. |
| Uniprot #: | SwissProt: O00585 Human |
| Alternative names: | 6Ckine Beta chemokine exodus 2 Beta-chemokine exodus-2 C C motif chemokine ligand 21 C-C motif chemokine 21 CCL21 CCL21_HUMAN Chemokine (C-C motif) ligand 21 Chemokine CC motif ligand 21 CKb9 ECL Efficient Chemoattractant for Lymphocytes SCYA21 Secondary lymphoid tissue chemokine Secondary lymphoid-tissue chemokine SLC Small inducible cytokine A21 Small inducible cytokine subfamily A (Cys-Cys), member 21 Small-inducible cytokine A21 TCA4 UNQ784/PRO1600 |
Images
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Fig1:
Sandwich ELISA analysis of human CCL21 matched pair antibodies Capture: HA723518, Human CCL21 Rabbit mAb [PSH13-36] Detector: HA723519, Human CCL21 Rabbit mAb [PSH13-37] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723518) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL21 protein (HA211144) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723519, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL21 in human cell culture supernatant samples. Capture: HA723518, Human CCL21 Rabbit mAb [PSH13-36] Detector: HA723519, Human CCL21 Rabbit mAb [PSH13-37] Interpolated concentration of native CCL21 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL21 concentration was determined to be 1,336 pg/mL in human serum. |
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Fig3:
Interpolated concentrations of spiked CCL21 in cell culture media samples. Capture: HA723518, Human CCL21 Rabbit mAb [PSH13-36] Detector: HA723519, Human CCL21 Rabbit mAb [PSH13-37] The concentrations of CCL21 were measured in duplicates, interpolated from the CCL21 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.