Human PD1 Recombinant Rabbit Monoclonal Antibody [PSH13-39]
cat.: HA723524
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PD1 protein aa 24-170 (HA210922). |
| Positive control: | Recombinant Human PD1 protein (HA210922). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [SJ01-91] to Human PD1 antibody (Capture) (HA723521) and recombinant Human PD1 protein (HA210922) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: Q15116 Human |
| Alternative names: | CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2 |
Images
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Fig1:
Sandwich ELISA analysis of Human PD1 matched pair antibodies Capture: HA723521, Human PD1 Rabbit mAb [SJ01-91] Detector: HA723524, Human PD1 Rabbit mAb [PSH13-39] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723521) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD1 protein (HA210922) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723524, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PD1 in MOLT-4 and HeLa extract samples based on a 1,000 µg/ml extract load. Capture: HA723521, Human PD1 Rabbit mAb [SJ01-91] Detector: HA723524, Human PD1 Rabbit mAb [PSH13-39] The concentrations of PD1 were measured in triplicates, interpolated from the PD-1 standard curve and corrected for sample dilution. Undiluted samples are MOLT-4 extract 100% and HeLa extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean PD1 concentration was determined to be 96.2 pg/ml in MOLT-4 extract and undetectable in HeLa extract. |
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Fig3:
Interpolated concentrations of native PD1 in human urine samples. Capture: HA723521, Human PD1 Rabbit mAb [SJ01-91] Detector: HA723524, Human PD1 Rabbit mAb [PSH13-39] The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 187.8 pg/ml in human urine. |
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Fig4:
Interpolated concentrations of spiked PD1 in human cell culture media samples. Capture: HA723521, Human PD1 Rabbit mAb [SJ01-91] Detector: HA723524, Human PD1 Rabbit mAb [PSH13-39] The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.