Mouse IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH13-41]
cat.: HA723527
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL1 alpha aa 115-270 (HA210971). |
| Positive control: | Recombinant Mouse IL-1 alpha protein (HA210971). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-40] to Mouse IL-1 alpha antibody (Capture) (HA723526) and recombinant Mouse IL-1 alpha protein (HA210971) as the standard. The reference range value is 4.9-1,200 pg/mL. |
| Uniprot #: | SwissProt: P01582 Mouse |
| Alternative names: | BAF FAF Hematopoietin 1 Hematopoietin-1 IL 1 alpha IL 1A IL-1 alpha Il-1a IL1 ALPHA IL1 IL1A IL1A_HUMAN IL1F1 Interleukin 1 alpha Interleukin-1 alpha Interleukin1 alpha LAF LEM Preinterleukin 1 alpha Pro interleukin 1 alpha |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723526) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723527, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,546 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant. |
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Fig3:
Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.