DCTN1 / p150-glued Recombinant Rabbit Monoclonal Antibody [PSH13-45]
cat.: HA723534
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IF-Cell, FC, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH13-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 142 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DCTN1 aa 1,051-1,278. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue, mouse brain tissue, HEK-293, Neuro-2a, PC-12. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, spindle, Nucleus envelope, cell cortex. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IF-Cell FC IF-Tissue IP |
1:15,000-1:50,000 1:500 1:1,000 1:100 1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q14203 Human | O08788 Mouse | P28023 Rat |
| Alternative names: | 150 kDa dynein associated polypeptide 150 kDa dynein-associated polypeptide DAP 150 DAP-150 DAP150 DCTN 1 DCTN1 DCTN1_HUMAN DP 150 DP-150 DP150 Dynactin 1 (p150 Glued (Drosophila) homolog) Dynactin 1 (p150 glued homolog Drosophila) Dynactin 1 Dynactin subunit 1 Dynactin1 HMN7B p135 p150 Glued (Drosophila) homolog p150 glued p150 glued homolog p150(GLUED) DROSOPHILA HOMOLOG OF p150-glued p150glued |
Images
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Fig1:
Western blot analysis of DCTN1 / p150-glued on different lysates with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/15,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723534) at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunocytochemistry analysis of HEK-293 cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig7:
Immunocytochemistry analysis of Neuro-2a cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Immunocytochemistry analysis of PC-12 cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig9:
Flow cytometric analysis of HEK-293 cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig10:
Flow cytometric analysis of Neuro-2a cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig11:
Flow cytometric analysis of PC-12 cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig12:
DCTN1 / p150-glued was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723534 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723534 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723534 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723534 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801 |
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