Bcl10 Recombinant Rabbit Monoclonal Antibody [PSH13-49]
cat.: HA723538
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC, IP
Clonality: Monoclonal
Clone number: PSH13-49
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Bcl10 aa 1-233.
Positive control: A20 cell lysate, RAW264.7 cell lysate, EL4 cell lysate, CTLL-2 cell lysate, Ramos cell lysate, Rat brain tissue lysate, Raji, RAW264.7.
Subcellular location: Cytoplasm, perinuclear region, Membrane raft.
Recommended Dilutions:
  WB
  IF-Cell
  FC
  IP
1:50,000
1:100
1:1,000
1-2μg/sample
Uniprot #: SwissProt: O95999 Human | Q9Z0H7 Mouse | Q9QYN5 Rat
Alternative names: AI132454 B cell CLL/lymphoma 10 B cell lymphoma/leukemia10 B-cell CLL/lymphoma 10 B-cell leukemia/lymphoma 10 B-cell lymphoma/leukemia 10 Bcl 10 Bcl-10 Bcl10 BCL10_HUMAN c E10 c-E10 C81403 CARD containing apoptotic signaling protein CARD containing molecule enhancing NF kappa B CARD containing molecule enhancing NF kB CARD containing molecule enhancing NF-kB CARD containing molecule enhancing NFkB CARD containing proapoptotic protein CARD like apoptotic protein CARD-containing apoptotic signaling protein CARD-containing molecule enhancing NF-kappa-B CARD-containing proapoptotic protein CARD-like apoptotic protein CARMEN Caspase recruiting domain containing protein caspase-recruiting domain-containing protein cCARMEN cE 10 cE10 CED 3/ICH 1 prodomain homologous E10 like regulator CED-3/ICH-1 prodomain homologous E10-like regulator CED3/ICH1 prodomain homologous E10 like regulator Cellular E10 Cellular homolog of vCARMEN ......
Images
HA723538_1.jpg Fig1: Western blot analysis of Bcl10 on different lysates with Rabbit anti-Bcl10 antibody (HA723538) at 1/50,000 dilution.

Lane 1: A20 cell lysate (20 µg/Lane)
Lane 2: RAW264.7 cell lysate (20 µg/Lane)
Lane 3: EL4 cell lysate (20 µg/Lane)
Lane 4: CTLL-2 cell lysate (20 µg/Lane)
Lane 5: Ramos cell lysate (20 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 26 kDa
Observed band size: 31 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723538) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723538_2.jpg Fig2: Immunocytochemistry analysis of Raji cells labeling Bcl10 with Rabbit anti-Bcl10 antibody (HA723538) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl10 antibody (HA723538) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723538_3.jpg Fig3: Immunocytochemistry analysis of RAW264.7 cells labeling Bcl10 with Rabbit anti-Bcl10 antibody (HA723538) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl10 antibody (HA723538) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA723538_4.jpg Fig4: Flow cytometric analysis of Raji cells labeling Bcl10.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723538, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723538_5.jpg Fig5: Flow cytometric analysis of RAW264.7 cells labeling Bcl10.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723538, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723538_6.jpg Fig6: Bcl10 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723538 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723538 at 1/50,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: RAW264.7 cell lysate (input)
Lane 2: HA723538 IP in RAW264.7 cell lysate
Lane 3: Rabbit IgG instead of HA723538 in RAW264.7 cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 20 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.