CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody [PSH13-51]
cat.: HA723540
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH13-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 262 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CACNA1E aa 1,964-2,313. |
| Positive control: | Mouse striatum tissue, mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:3,000-1:6,000 1:5,000 |
| Uniprot #: | SwissProt: Q61290 Mouse | Q07652 Rat |
| Alternative names: | alpha-1 polypeptide BII Brain calcium channel II CAC 1E CAC1E CAC1E_HUMAN CACH 6 CACH6 CACNA1E Cacnl1 a6 CACNL1A6 Calcium channel Calcium channel, L type, alpha 1 polypeptide, isoform 6 calcium channel, R type Cav 2.3 Cchra 1 Cchra1 isoform 6 L type Voltage dependent R type calcium channel subunit alpha 1E Voltage gated calcium channel subunit alpha Cav2.3 Voltage-dependent R-type calcium channel subunit alpha-1E Voltage-gated calcium channel subunit alpha Cav2.3 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723540) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody (HA723540) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse hippocampus tissue lysate (no heat) Lane 3: Mouse lung tissue lysate (negative) (no heat) Lane 4: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 262 kDa Observed band size: 257 kDa Exposure time: 46 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723540) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.