BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52]
cat.: HA723541
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH13-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BATF3 aa 1-127. |
| Positive control: | U-937 cell lysate, HDLM2 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP ChIP |
1:5,000 1-2μg/sample Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: Q9NR55 Human |
| Alternative names: | 21 kD small nuclear factor isolated from T cells 21 kDa small nuclear factor isolated from T-cells ATF like 3 B-ATF-3 basic leucine zipper transcription factor, ATF-like 3 BATF3 FLJ36352 FLJ37535 hCG 39202 JDP1 Jun dimerization protein 1 Jun dimerization protein p21SNFT JUNDM1 SNFT |
Images
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Fig1:
Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA723541) at 1/5,000 dilution. Lane 1: U-937 cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: HDLM2 cell lysate Lane 4: HepG2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723541) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA723541 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723541 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-937 cell lysate (input) Lane 2: HA723541 IP in U-937 cell lysate Lane 3: Rabbit IgG instead of HA723541 in U-937 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801 |
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Fig3: Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA723541) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.