AMACR Recombinant Rabbit Monoclonal Antibody [PSH13-54]
cat.: HA723543
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH13-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AMACR aa 1-382. |
| Positive control: | LNCaP cell lysate, U-2 OS cell lysate, A375 cell lysate, Mouse kidney tissue lysate, human prostate cancer tissue, human renal cell carcinoma tissue, human kidney tissue. |
| Subcellular location: | Peroxisome, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:10,000 1:1,000 |
| Uniprot #: | SwissProt: Q9UHK6 Human | O09174 Mouse |
| Alternative names: | 2 arylpropionyl CoA epimerase 2 methylacyl CoA racemase 2-methylacyl-CoA racemase Alpha methylacyl CoA racemase Alpha methylacyl Coenzyme A racemase Alpha methylacyl-CoA racemase deficiency, included Alpha-methylacyl-CoA racemase Amacr AMACR deficiency, included AMACR_HUMAN CBAS4 Da1-8 EC 5.1.99.4 Macr1 Methylacyl CoA racemase alpha RACE RM P504S |
Images
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Fig1:
Western blot analysis of AMACR on different lysates with Rabbit anti-AMACR antibody (HA723543) at 1/5,000 dilution. Lane 1: LNCaP cell lysate (20 µg/Lane) Lane 2: U-2 OS cell lysate (low expression) (20 µg/Lane) Lane 3: A375 cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minute 22 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723543) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (HA723543) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723543) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: IF-Tissue Species: Human Site: kidney Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.