MICA Recombinant Rabbit Monoclonal Antibody [PSH13-60]
cat.: HA723549
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH13-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MICA aa 1-328. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, OVCAR-3 cell lysate, HeLa. |
| Subcellular location: | Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB FC IP |
1:5,000 1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q29983 Human |
| Alternative names: | HLA class I antigen FLJ36918 FLJ60820 MGC111087 MGC21250 MHC class I chain related gene A protein MHC class I chain related protein A MHC class I chain related protein A HLA B HLA C MHC class I polypeptide related sequence A MHC class I polypeptide-related sequence A MHC class I related protein MIC A MIC-A micA MICA_HUMAN OTTHUMP00000029088 OTTHUMP00000044528 OTTHUMP00000165170 OTTHUMP00000165172 PERB11.1 Stress inducible class I homolog |
Images
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Fig1:
Western blot analysis of MICA on different lysates with Rabbit anti-MICA antibody (HA723549) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: OVCAR-3 cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723549) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723549, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
MICA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723549 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723549 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723549 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723549 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.