Phospho-PLC-gamma-1 (S1248) Recombinant Rabbit Monoclonal Antibody [PSH13-62]
cat.: HA723551
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH13-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLC-gamma-1. |
| Positive control: | A431 cell lysate, A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate. |
| Subcellular location: | Cell projection, lamellipodium, ruffle. |
| Recommended Dilutions:
WB IP |
1:2,000-1:10,000 1-2μg/sample |
| Uniprot #: | SwissProt: P19174 Human |
| Alternative names: | 1 phosphatidyl D myo inositol 4 5 bisphosphate 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase NCKAP3 Phosphatidylinositol phospholipase C Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-1 Phospholipase C 148 Phospholipase C gamma 1 Phospholipase C-gamma-1 Phospholipase C-II PLC gamma 1 PLC II PLC-148 PLC-gamma-1 PLC-II PLC1 PLC148 Plcg1 PLCG1_HUMAN PLCgamma1 |
Images
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Fig1:
Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody (HA723551) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723551) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with HA723551 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723551 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input) Lane 2: HA723551 IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: Rabbit IgG instead of HA723551 in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.