Mouse CD86 Recombinant Rabbit Monoclonal Antibody [PSH13-66]
cat.: HA723556
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD68 aa 24-244 (HA210879). |
| Positive control: | Recombinant Mouse CD86 protein (HA210879). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-65] to Mouse CD86 antibody (Capture) (HA723555) and recombinant Mouse CD86 protein (HA210879) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: P42082 Mouse |
| Alternative names: | Activation B7-2 antigen 3 Activation B7-2 antigen B-lymphocyte activation antigen B7-2 2 B-lymphocyte activation antigen B7-2 B7 2 B7 B7-2 B7.2 B70 B72 B72 antigen BU63 CD28 antigen ligand 2 2 CD28 antigen ligand 2 Cd28l2 CD28LG2 CD86 CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) CD86 antigen CD86 molecule CD86_HUMAN CLS1 CTLA-4 counter-receptor B7.2 2 CTLA-4 counter-receptor B7.2 2, 3 CTLA-4 counter-receptor B7.2 Early T-cell costimulatory molecule 1 ETC-1 FUN 1 FUN-1 FUN1 LAB72 Ly-58 Ly58 MB7 MB7-2 Membrane glycoprotein MGC34413 T lymphocyte activation antigen CD86 precursor T-lymphocyte activation antigen CD86 TS/A-2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723555) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723556, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 263 pg/mL in Mouse serum. |
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Fig3:
Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 181 pg/mL in J774A.1 Cell extract. |
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Fig4:
Interpolated concentrations of spiked CD86 in cell culture media samples. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] The concentrations of CD86 were measured in duplicates, interpolated from the CD86 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.