Caspase-7 Recombinant Rabbit Monoclonal Antibody [PSH13-69]
cat.: HA723558
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH13-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Caspase-7 aa 1-303. |
| Positive control: | HeLa cell lysate, HeLa treated with 1μM staurosporine for 3 hours cell lysate, C2C12 cell lysate, C2C12 treated with 1μM staurosporine for 3 hours cell lysate, human colon tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus, Secreted, extracellular space. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P55210 Human | P97864 Mouse Entrez Gene: 64026 Rat |
| Alternative names: | Apoptotic protease Mch-3 Apoptotic protease MCH3 CASP-7 CASP7 CASP7_HUMAN Caspase 7 Caspase 7 apoptosis related cysteine peptidase Caspase-7 subunit p11 Caspase7 CMH 1 CMH-1 CMH1 ICE LAP3 ICE-LAP3 ICE-like apoptotic protease 3 LICE2 MCH3 |
Images
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Fig1:
Western blot analysis of Caspase-7 on different lysates with Rabbit anti-Caspase-7 antibody (HA723558) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM staurosporine for 3 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 1μM staurosporine for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34/32/20/18 kDa Exposure time: 2 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723558) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Caspase-7 antibody (HA723558) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723558) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Caspase-7 antibody (HA723558) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Caspase-7 antibody (HA723558) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.