HRP Conjugated Human IGFBP3 Recombinant Rabbit Monoclonal Antibody [PSH12-17]
cat.: HA723565H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH12-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IGFBP3 aa 28-291. |
| Positive control: | Recombinant Human IGFBP3 protein (HA210585). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-16] to Human IGFBP3 antibody (Capture) (HA723432) and Recombinant Human IGFBP3 protein (HA210585) as the standard. |
| Uniprot #: | SwissProt: P17936 Human |
| Alternative names: | Acid stable subunit of the 140 K IGF complex Binding protein 29 Binding protein 53 BP 53 BP53 Growth hormone dependent binding protein IBP 3 IBP-3 IBP3 IBP3_HUMAN IGF binding protein 3 IGF-binding protein 3 IGFBP 3 IGFBP-3 IGFBP3 Insulin Like Growth Factor Binding Protein 3 Insulin-like growth factor binding protein 3 precursor Insulin-like growth factor-binding protein 3 |
Images
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Fig1:
Sandwich ELISA analysis of human IGFBP3 matched pair antibodies Capture: HA723432, Human Leptin Rabbit mAb [PSH12-16] Detector: HA723433, Human Leptin Rabbit mAb [PSH12-17] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723432) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IGFBP3 protein (HA210585) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723433, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.