Human Lipoprotein A Recombinant Rabbit Monoclonal Antibody [PSH13-81]
cat.: HA723570
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LPA aa 20-1,033 (HA210979). |
| Positive control: | Recombinant Human Lipoprotein A protein (HA210979). |
| Subcellular location: | Extracellular region, plasma lipoprotein particle. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-82] to Human Lipoprotein A antibody (Detector) (HA723571) or Rabbit monoclonal [PSH13-83] to Human Lipoprotein A antibody (Detector) (HA723573) and recombinant Human Lipoprotein A protein (HA210979) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P08519 Human |
| Alternative names: | AK38 Antiangiogenic AK38 protein apo(a) APOA apolipoprotein(a) LP lp(a) LPA |
Images
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Fig1:
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723571, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723573, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native Lipoprotein A in human urine samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 5,193 pg/ml in human urine. |
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Fig4:
Interpolated concentrations of native Lipoprotein A in human urine samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4,023 pg/ml in human urine. |
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Fig5:
Interpolated concentrations of native Lipoprotein A in human serum samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.033%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4.48 µg/ml in human serum. |
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Fig6:
Interpolated concentrations of native Lipoprotein A in human serum samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.004%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 16.01 µg/ml in human serum. |
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Fig7:
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig8:
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples. Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83] The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.