HRP Conjugated Human Lipoprotein A Recombinant Rabbit Monoclonal Antibody [PSH13-83]
cat.: HA723574H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LPA aa 20-1,033 (HA210979). |
| Positive control: | Recombinant Human Lipoprotein A protein (HA210979). |
| Subcellular location: | Extracellular region, plasma lipoprotein particle. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-81] to Human Lipoprotein A antibody (Capture) (HA723570) and recombinant Human Lipoprotein A protein (HA210979) as the standard. The reference range value is 1,370-37,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P08519 Human |
| Alternative names: | AK38 Antiangiogenic AK38 protein apo(a) APOA apolipoprotein(a) LP lp(a) LPA |
Images
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Fig1:
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81] Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 37,000 pg/ml to 0 pg/ml and detect antibody (HA723573, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.