Biotin Conjugated Human CD62L Recombinant Rabbit Monoclonal Antibody [PSH13-86]
cat.: HA723579B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH13-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD62L aa 39-332 (HA211181). |
| Positive control: | Recombinant Human CD62L protein (HA211181). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-85] to Human CD62L antibody (Capture) (HA723576) and Recombinant Human CD62L protein (HA211181) as the standard. The reference range value is 19.5-5,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P14151 Human |
| Alternative names: | A.11 AI528707 CD62 antigen ligand CD62 antigen-like family member L CD62L gp90-MEL IgA nephropathy, susceptibility to, included L Selectin L-selectin LAM-1 LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu-8 Leukocyte-endothelial cell adhesion molecule 1 Lnhr LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 LYAM1_HUMAN Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen MEL-14 Pln homing receptor PLNHR Selectin L Selectin, lymphocyte SELL TQ1 |
Images
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Fig1:
Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723576) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723578, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD62L in human samples. Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,792 pg/mL in Jurkat cell supernatant. There was no detectable signal in Hela cell supernatant. |
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Fig3:
Interpolated concentrations of spiked CD62L in cell culture media samples. Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] The concentrations of CD62L were measured in duplicates, interpolated from the CD62L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.