DR3 / LARD Recombinant Rabbit Monoclonal Antibody [PSH13-90]
cat.: HA723583
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH13-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DR3 / LARD aa 1-199. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, THP-1 cell lysate, HDLM-2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: Q93038 Human Entrez Gene: 85030 Mouse | 500592 Rat |
| Alternative names: | Apo 3 Apo-3 Apo3 Apoptosis inducing receptor AIR Apoptosis inducing receptor Apoptosis mediating receptor Apoptosis mediating receptor DR 3 Apoptosis mediating receptor DR3 Apoptosis mediating receptor TRAMP Apoptosis-inducing receptor AIR Apoptosis-mediating receptor DR3 Apoptosis-mediating receptor TRAMP DDR 3 DDR3 Death domain receptor 3 Death domain receptor 3 soluble form Death receptor 3 Death receptor beta DR 3 DR3 LARD Lymphocyte associated receptor of death Lymphocyte-associated receptor of death Protein WSL Protein WSL-1 TNF receptor superfamily member 25 TNFR25 TNFRSF 12 TNFRSF 25 TNFRSF12 TNFRSF12, formerly TNFRSF25 TNR25_HUMAN TR 3 TR3 TRAMP Translocating chain association membrane protein Tumor necrosis factor receptor superfamily member 12 Tumor necrosis factor receptor superfamily member 25 Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein)...... |
Images
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Fig1:
Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HDLM-2 cell lysate (20 µg/Lane) Lane 5: U-87 MG cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.