QKI-6 Recombinant Antibody [PSH10-23] - Rabbit IgG (Chimeric)
cat.: HA723588
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH10-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Immunogen: | Synthetic peptide within human QKI-6 aa 281-319 / 319. |
| Positive control: | Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:5,000 1:5,000 |
| Uniprot #: | SwissProt: Q96PU8-9 Human | Q9QYS9 Mouse | Q91XU1 Rat |
| Alternative names: | DKFZp586I0923 HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI OTTHUMP00000017581 OTTHUMP00000017582 OTTHUMP00000017583 Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK QKI-6 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 (QKI-6, HA723588, Rabbit, green); 1/500 (NF-M, HA601443, Mouse, red) Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 (QKI-6, HA723588, Rabbit, green); 1/500 (NF-M, HA601444, Guinea pig, red) Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 (QKI-6, HA723588, Rabbit, green); 1/500 (NF-M, HA601443, Mouse, red) Antigen retrieval: Not required |
|
Fig4:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 (QKI-6, HA723588, Rabbit, green); 1/500 (NF-M, HA601444, Guinea pig, red) Antigen retrieval: Not required |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-6 antibody (HA723588) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723588) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of QKI-6 on different lysates with Rabbit anti-QKI-6 antibody (HA723588) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.