Biotin Conjugated Human TIM 4 Recombinant Rabbit Monoclonal Antibody [PSH14-01]
cat.: HA723591B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH14-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TIM 4 aa 25-314 (HA211014). |
| Positive control: | Recombinant Human TIM 4 protein (HA211014). |
| Subcellular location: | Cell membrane, Secreted, extracellular exosome. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-00] to Human TIM 4 antibody (Capture) (HA723589) and recombinant Human TIM 4 protein (HA211014) as the standard. The reference range value is 11.7-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q96H15 Human |
| Alternative names: | SMUCKLER Spleen, mucin-containing, knockout of lymphotoxin protein T cell immunoglobulin and mucin domain containing protein 4 T-cell immunoglobulin and mucin domain containing 4 T-cell immunoglobulin and mucin domain containing molecule T-cell immunoglobulin and mucin domain-containing protein 4 T-cell immunoglobulin and mucin domains-containing protein 4 T-cell membrane protein 4 TIM-4 Tim4 TIMD 4 TIMD-4 Timd4 TIMD4_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human TIM 4 matched pair antibodies Capture: HA723589, Human TIM 4 Rabbit mAb [PSH14-00] Detector: HA723590, Human TIM 4 Rabbit mAb [PSH14-01] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723589) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TIM 4 protein (HA211014) protein starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA723590, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of spiked TIM 4 in cell culture media samples. Capture: HA723589, Human TIM 4 Rabbit mAb [PSH14-00] Detector: HA723590, Human TIM 4 Rabbit mAb [PSH14-01] The concentrations of TIM 4 were measured in duplicates, interpolated from the TIM 4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.