PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06]
cat.: HA723596
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PRAK / MK5 aa 251-473. |
| Positive control: | HeLa cell lysate, A431 cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, Jurkat cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q8IW41 Human | O54992 Mouse Entrez Gene: 498183 Rat |
| Alternative names: | MAP kinase-activated protein kinase 5 MAPK-activated protein kinase 5 MAPK5_HUMAN MAPKAP kinase 5 MAPKAPK-5 MAPKAPK5 Mitogen activated protein kinase activated protein kinase 5 p38-regulated/activated protein kinase PRAK |
Images
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Fig1:
Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA723596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: Jurkat cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: MEF cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 52/66 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723596 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723596 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723596 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723596 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.