NDUFA9 Recombinant Rabbit Monoclonal Antibody [PSH14-07]
cat.: HA723597
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDUFA9 aa 1-230. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Caco-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human colon cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q16795 Human | Q9DC69 Mouse | Q5BK63 Rat |
| Alternative names: | CI-39kD Complex I subunit NDUFA9 Complex I-39kD mitochondrial NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 NADH-ubiquinone oxidoreductase 39 kDa subunit NDUA9_HUMAN NDUFA9 |
Images
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Fig1:
Western blot analysis of NDUFA9 on different lysates with Rabbit anti-NDUFA9 antibody (HA723597) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Caco-2 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: Mouse liver tissue lysate Lane 11: Rat liver tissue lysate Lane 12: Mouse skeletal muscle tissue lysate Lane 13: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 36 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723597) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-NDUFA9 antibody (HA723597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723597) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFA9 antibody (HA723597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723597) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NDUFA9 antibody (HA723597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723597) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NDUFA9 antibody (HA723597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723597) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
NDUFA9 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723597 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723597 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723597 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723597 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 7 seconds; ECL: K1801 |
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