NDUFA9 Recombinant Rabbit Monoclonal Antibody [PSH14-08]
cat.: HA723598
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDUFA9 aa 1-230. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Caco-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, L6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q16795 Human | Q9DC69 Mouse | Q5BK63 Rat |
| Alternative names: | CI-39kD Complex I subunit NDUFA9 Complex I-39kD mitochondrial NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 NADH-ubiquinone oxidoreductase 39 kDa subunit NDUA9_HUMAN NDUFA9 |
Images
|
Fig1:
Western blot analysis of NDUFA9 on different lysates with Rabbit anti-NDUFA9 antibody (HA723598) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Caco-2 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Neuro-2a cell lysate Lane 8: C6 cell lysate Lane 9: L6 cell lysate Lane 10: Mouse liver tissue lysate Lane 11: Rat liver tissue lysate Lane 12: Mouse skeletal muscle tissue lysate Lane 13: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 36 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723598) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
NDUFA9 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723598 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723598 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723598 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723598 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 15 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.