TGF beta Receptor I Recombinant Rabbit Monoclonal Antibody [PSH14-14]
cat.: HA723604
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TGF beta Receptor I aa 34-503. |
| Positive control: | THP-1 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, C6 cell lysate. |
| Subcellular location: | Cell membrane, Cell junction, tight junction, Cell surface, Membrane raft. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P36897 Human | Q64729 Mouse | P80204 Rat |
| Alternative names: | AAT 5 AAT5 Activin A receptor type II like kinase 53kDa Activin A receptor type II like kinase Activin A receptor type II like kinase, 53kD Activin A receptor type II like protein kinase of 53kD activin A receptor type II-like kinase, 53kDa activin A receptor type II-like protein kinase of 53kD Activin receptor like kinase 5 Activin receptor-like kinase 5 ACVRLK 4 ACVRLK4 ALK 5 ALK-5 ALK5 LDS1A LDS2A MSSE Serine/threonine protein kinase receptor R4 Serine/threonine-protein kinase receptor R4 SKR 4 SKR4 TbetaR I TbetaR-I TGF beta receptor type 1 TGF beta receptor type I TGF beta type I receptor TGF-beta receptor type I TGF-beta receptor type-1 TGF-beta type I receptor TGFBR 1 TGFBR1 TGFBR1 protein TGFR 1 TGFR-1 TGFR1 TGFR1_HUMAN Transforming growth factor beta receptor 1 Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) Transforming growth factor beta receptor I transf...... |
Images
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Fig1:
Western blot analysis of TGF beta Receptor I on different lysates with Rabbit anti-TGF beta Receptor I antibody (HA723604) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF7 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Neuro-2a cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50/37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723604) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
TGF beta Receptor I was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA723604 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723604 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: HA723604 IP in THP-1 cell lysate Lane 3: Rabbit IgG instead of HA723604 in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.