FLI1 Recombinant Rabbit Monoclonal Antibody [PSH14-17]
cat.: HA723605
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FLI1 aa 351-400 / 452. |
| Positive control: | U-937 cell lysate, Jurkat cell lysate, human B cell lymphoma tissue, human lung tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q01543 Human |
| Alternative names: | ERGB transcription factor Ewing Sarcoma breakpoint region 2 EWSR 2 EWSR2 FLI 1 FLI 1 proto oncogene Fli-1 proto-oncogene, ETS transcription factor FLI1 FLI1 EWS fusion gene FLI1 proto oncogene FLI1_HUMAN Friend leukemia integration 1 (FLI1) transcription factor Friend leukemia integration 1 transcription factor Friend leukemia virus integration 1 Proto-oncogene Fli-1 Sarcoma breakpoint region 2 (EWSR2) SIC 1 SIC1 Transcription factor ERGB Viral integration region FLI1 Viral integration region FLI1, mouse, homolog of |
Images
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Fig1:
Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution. Lane 1: U-937 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 2 minutes 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-FLI1 antibody (HA723605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.