Human Antithrombin III / ATIII Recombinant Rabbit Monoclonal Antibody [PSH14-25]
cat.: HA723615
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Antithrombin III aa 33-464 (HA210985). |
| Positive control: | Recombinant Human Antithrombin III protein (HA210985). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-26] to Human Antithrombin III antibody (Detector) (HA723616) and Recombinant Human Antithrombin III protein (HA210985) as the standard. The reference range value is 39.1-10,000 pg/ml. |
| Uniprot #: | SwissProt: P01008 Human |
| Alternative names: | ANT3_HUMAN Antithrombin Antithrombin III Antithrombin-III AntithrombinIII AT 3 AT III AT3 AT3D ATIII Heparin cofactor I MGC22579 Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 Serine cysteine proteinase inhibitor clade C member 1 Serine proteinase inhibitor clade C member 1 Serpin C1 Serpin family C member 1 Serpin peptidase inhibitor clade C (antithrombin) member 1 SERPINC1 THPH7 |
Images
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Fig1:
Sandwich ELISA analysis of Human Antithrombin III matched pair antibodies Capture: HA723615, Human Antithrombin III Rabbit mAb [PSH14-25] Detector: HA723616, Human Antithrombin III Rabbit mAb [PSH14-26] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723615) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Antithrombin III protein (HA210985) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723616, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Antithrombin III in HepG2 culture supernatant and Human urine. Capture: HA723615, Human Antithrombin III Rabbit mAb [PSH14-25] Detector: HA723616, Human Antithrombin III Rabbit mAb [PSH14-26] Interpolated concentration of native Antithrombin III was measured in duplicate at different sample concentrations and interpolated from the Antithrombin III standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 25046 pg/ml in HepG2 cell culture supernatant,317 pg/ml in Human urine. |
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Fig3:
Interpolated concentrations of spiked Antithrombin III in cell culture media samples. Capture: HA723615, Human Antithrombin III Rabbit mAb [PSH14-25] Detector: HA723616, Human Antithrombin III Rabbit mAb [PSH14-26] The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.