Mouse IL-27A p28 Recombinant Rabbit Monoclonal Antibody [PSH14-33]
cat.: HA723627
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-27A p28 aa 29-234 (HA211005). |
| Positive control: | Recombinant Mouse IL-27A p28 protein (HA211005). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-32] to Mouse IL-27A p28 antibody (Detector) (HA723625) and Recombinant Mouse IL-27A p28 protein (HA211005) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: Q8K3I6 Mouse |
| Alternative names: | EBI3 IL 27 IL 27 p28 subunit IL 27A IL 30 IL-27 subunit alpha IL-27-A IL27 IL27-A IL27A IL27A_HUMAN IL27p28 IL30 Interleukin 27 Interleukin 27 subunit alpha Interleukin 27, 28-KD subunit Interleukin 30 Interleukin-27 subunit alpha p28 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723627) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-27A p28 in LPS stimulated RAW 264.7 cell culture supernatant. Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW 264.7 cells were cultured for 48 hours and stimulated with 5 µg/ml LPS. The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are stimulated RAW 264.7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-27A p28 concentration was to be 1,264 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native IL-27A p28 in RAW 264.7 cell culture supernatant. Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW2 64.7 were stimulated with 5 µg/ml LPS and incubated for 48 hours. The concentrations of IL-27A p28 were interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are LPS stimulated RAW 264.7 cell culture supernatant 50% and unstimulated RAW 264.7 cell culture supernatant 50%. The mean IL-27A p28 concentration was determined to be 1,264 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant and undetectable in the unstimulated RAW 264.7 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked IL-27A p28 in human cell culture media samples. Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.