ALP Recombinant Rabbit Monoclonal Antibody [PSH14-35]
cat.: HA723629
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ALP aa 1-364. |
| Positive control: | Saos-2 cell lysate, RD cell lysate, U-2 OS cell lysate, 293T cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Cytoplasm, myofibril, sarcomere, Z line. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q53GG5 Human | O70209 Mouse | Q66HS7 Rat |
| Alternative names: | Actinin associated LIM protein Actinin-associated LIM protein Alpha actinin 2 associated LIM protein Alpha-actinin-2-associated LIM protein DKFZp686L0362 Enigma homolog PDLI3_HUMAN PDLIM 3 PDLIM3 PDZ and LIM domain 3 PDZ and LIM domain protein 3 |
Images
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Fig1:
Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA723629) at 1/5,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: RD cell lysate Lane 3: U-2 OS cell lysate Lane 4: 293T cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate (negative) Lane 9: Rat heart tissue lysate Lane 10: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723629) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
ALP was immunoprecipitated from 0.2 mg Saos-2 cell lysate with HA723629 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723629 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Saos-2 cell lysate (input) Lane 2: HA723629 IP in Saos-2 cell lysate Lane 3: Rabbit IgG instead of HA723629 in Saos-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.