HUWE1 Recombinant Rabbit Monoclonal Antibody [PSH14-37]
cat.: HA723631
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH14-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 482 kDa
Isotype: IgG
Immunogen: Recombinant protein within human HUWE1 aa 3,950-4,374.
Positive control: HeLa cell lysate, SW480 cell lysate, A549 cell lysate, MOLT-4 cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, F9 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue.
Subcellular location: Cytoplasm, Nucleus, Mitochondrion.
Recommended Dilutions:
  WB
  IHC-P
1:5,000-1:20,000
1:50-1:100
Uniprot #: SwissProt: Q7Z6Z7 Human | Q7TMY8 Mouse | P51593 Rat
Alternative names: ARF binding protein 1 ARF BP1 ARF-binding protein 1 ARF-BP1 ARFBP1 BJ-HCC-24 tumor antigen E3 ubiquitin protein ligase HUWE1 E3 ubiquitin-protein ligase HUWE1 HECT HECT domain protein LASU1 HECT UBA and WWE domain containing protein 1 HectH9 Homologous to E6AP carboxyl terminus homologous protein 9 Huwe1 HUWE1_HUMAN Ib772 Large structure of UREB1 LASU1 Mcl 1 ubiquitin ligase E3 Mcl-1 ubiquitin ligase E3 Mule UBA and WWE domain-containing protein 1 Upstream regulatory element-binding protein 1 URE-B1 URE-binding protein 1 UREB1
Images
HA723631_1.jpg Fig1: Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (low expression) (20 µg/Lane)
Lane 4: MOLT-4 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: F9 cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)

Predicted band size: 482 kDa
Observed band size: 482 kDa

Exposure time: 25 seconds; ECL: K1801;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723631_2.jpg Fig2: Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/20,000 dilution.

Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 482 kDa
Observed band size: 482 kDa

Exposure time: 48 seconds; ECL: K1801;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723631_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723631_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723631_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723631_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723631_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA723631_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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