HRP Conjugated Human CD62E Recombinant Rabbit Monoclonal Antibody [PSH14-42]
cat.: HA723637H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH14-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD62E aa 22-556 (HA210996). |
| Positive control: | Recombinant Human CD62E protein (HA210996). |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-41] to Human CD62E antibody (Capture) (HA723635) and Recombinant Human CD62E protein (HA210996) as the standard. The reference range value is 625-40,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P16581 Human |
| Alternative names: | CD26E CD62 antigen-like family member E CD62E E selectin E-selectin ELAM ELAM-1 ELAM1 Endothelial adhesion molecule 1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN Sele Selectin E |
Images
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Fig1:
Sandwich ELISA analysis of Human CD62E matched pair antibodies Capture: HA723635, Human CD62E Rabbit mAb [PSH14-41] Detector: HA723636, Human CD62E Rabbit mAb [PSH14-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723635) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62E protein (HA210996) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA723636, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.