Human alpha Fetoprotein Recombinant Rabbit Monoclonal Antibody [PSH14-55]
cat.: HA723647
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AFP aa 19-609 (HA210967). |
| Positive control: | Recombinant Human alpha Fetoprotein protein (HA210967). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-53] to Human alpha Fetoprotein antibody (Capture) (HA723644) and Recombinant Human alpha Fetoprotein protein (HA210967) as the standard. The reference range value is 39-10,000 pg/mL. |
| Uniprot #: | SwissProt: P02771 Human |
| Alternative names: | Afp AFPD Alpha fetoglobulin Alpha fetoprotein Alpha fetoprotein precursor Alpha-1-fetoprotein Alpha-fetoglobulin Alpha-fetoprotein alpha-fetoprotein, Hereditary persistence of, included FETA FETA_HUMAN Hereditary persistence of alpha fetoprotein HPAFP |
Images
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Fig1:
Sandwich ELISA analysis of Human alpha Fetoprotein matched pair antibodies Capture: HA723644, Human alpha Fetoprotein Rabbit mAb [PSH14-53] Detector: HA723647, Human alpha Fetoprotein Rabbit mAb [PSH14-55] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723644) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human alpha Fetoprotein protein (HA210967) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723647, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Alpha-fetoprotein in human samples. Capture: HA723644, Human alpha Fetoprotein Rabbit mAb [PSH14-53] Detector: HA723647, Human alpha Fetoprotein Rabbit mAb [PSH14-55] Interpolated concentration of native Alpha-fetoprotein was measured in duplicate at different sample concentrations and interpolated from the Alpha-fetoprotein standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Alpha-fetoprotein concentration was determined to be 1.8 ug/mL in Hep G2 cell supernatant. There was no detectable signal in THP-1 cell supernatant. |
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Fig3:
Interpolated concentrations of spiked Alpha-fetoprotein in cell culture media samples. Capture: HA723644, Human alpha Fetoprotein Rabbit mAb [PSH14-53] Detector: HA723647, Human alpha Fetoprotein Rabbit mAb [PSH14-55] The concentrations of Alpha-fetoprotein were measured in duplicates, interpolated from the Alpha-fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.