Mouse IL-21 Recombinant Rabbit Monoclonal Antibody [PSH04-75]
cat.: HA723649
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-21 aa 25-146 (HA210801). |
| Positive control: | Recombinant Mouse IL-21 protein (HA210801). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-56] to Mouse IL-21 antibody (Detector) (HA723650) or Rabbit monoclonal [PSH14-57] to Mouse IL-21 antibody (Detector) (HA723652) and Recombinant Mouse IL-21 protein (HA210801) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q9ES17 Mouse |
| Alternative names: | CVID11 IL 21 IL-21 Il21 IL21_HUMAN Interleukin 21 Interleukin-21 interleukin-21 isoform Interleukin21 OTTHUMP00000164088 Za11 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IL-21 matched pair antibodies Capture: HA723649, Mouse IL-21 Rabbit mAb [PSH04-75] Detector: HA723650, Mouse IL-21 Rabbit mAb [PSH14-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723649) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted IL-21 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723650, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Mouse IL-21 matched pair antibodies Capture: HA723649, Mouse IL-21 Rabbit mAb [PSH04-75] Detector: HA723652, Mouse IL-21 Rabbit mAb [PSH14-57] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723649) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-21 protein (HA210801) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723652, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of spiked IL-21 in mouse cell culture media samples. Capture: HA723649, Mouse IL-21 Rabbit mAb [PSH04-75] Detector: HA723650, Mouse IL-21 Rabbit mAb [PSH14-56] The concentrations of IL-21 were measured in duplicates, interpolated from the IL-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked IL-21 in mouse cell culture media samples. Capture: HA723649, Mouse IL-21 Rabbit mAb [PSH04-75] Detector: HA723652, Mouse IL-21 Rabbit mAb [PSH14-57] The concentrations of IL-21 were measured in duplicates, interpolated from the IL-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.