HA723660B

Biotin Conjugated Human Resistin Recombinant Rabbit Monoclonal Antibody [PSH14-62]

cat.: HA723660B

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Cynomolgus monkey
Applications:	ELISA(Det), ELISA
Clonality:	Monoclonal
Clone number:	PSH14-62

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Isotype:	IgG
Immunogen:	Recombinant protein within Human Resistin aa 17-108 (HA211002).
Positive control:	Recombinant Human Resistin protein (HA211002).
Subcellular location:	Secreted.
Recommended Dilutions: ELISA(Det) ELISA	Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-61] to Human Resistin antibody (Capture) (HA723658) or Rabbit monoclonal [PSH14-63] to Human Resistin antibody (Capture) (HA723661) and Recombinant Human Resistin protein (HA211002) as the standard. The reference range value is 7.8-2,000 pg/mL. Use at an assay dependent concentration.
Uniprot #:	SwissProt: Q9HD89 Human
Alternative names:	Adipose tissue specific secretory factor Adipose tissue-specific secretory factor ADSF C/EBP epsilon regulated myeloid specific secreted cysteine rich protein C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein CG5403 Cysteine rich secreted protein A12 alpha like 2 Cysteine rich secreted protein FIZZ3 Cysteine-rich secreted protein A12-alpha-like 2 Cysteine-rich secreted protein FIZZ3 dri FIZZ 3 FIZZ3 Found in inflammatory zone 3 HXCP 1 HXCP1 MGC126603 MGC126609 PRO1199 Resistin Resistin delta2 RETN 1 RETN RETN_HUMAN RETN1 RSTN UNQ407 XCP 1 XCP1

Images

Fig1: Sandwich ELISA analysis of Human Resistin matched pair antibodies

Capture: HA723658, Human Resistin Rabbit mAb [PSH14-61]
Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723658) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Resistin protein (HA211002) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723659, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Fig2: Sandwich ELISA analysis of Human Resistin matched pair antibodies

Capture: HA723661, Human Resistin Rabbit mAb [PSH14-63]
Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723661) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Resistin protein (HA211002) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723659, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

	Fig3: Interpolated concentrations of native Resistin in human samples. Capture: HA723658, Human Resistin Rabbit mAb [PSH14-61] Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the Resistin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 3,428 pg/mL in U937 cell supernatant. There was no detectable signal in THP-1 cell supernatant.
	Fig4: Interpolated concentrations of native Resistin in human samples. Capture: HA723661, Human Resistin Rabbit mAb [PSH14-63] Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the Resistin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 3,917 pg/mL in U937 cell supernatant. There was no detectable signal in THP-1 cell supernatant.
	Fig5: Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723658, Human Resistin Rabbit mAb [PSH14-61] Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
	Fig6: Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723661, Human Resistin Rabbit mAb [PSH14-63] Detector: HA723659, Human Resistin Rabbit mAb [PSH14-62] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.