Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70]
cat.: HA723668
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 166 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Homeobox protein cut-like 1 aa 1,000-1,200. |
| Positive control: | Mouse cerebrum tissue, rat cerebrum tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:200-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P53564 Mouse | P53565 Rat |
| Alternative names: | CCAAT displacement protein CDP CDP/Cut CDP1 Clox COY1 Cut homeobox Cut homolog Cut like 1 Cut like homeobox 1 CUTL1 CUX Cux/CDP CUX1 Golgi integral membrane protein 6 GOLIM6 Homeobox protein cux 1 Nbla10317 p100 p110 p200 p75 Putative protein product of Nbla10317 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebrum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Rat Site: cerebrum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA723668) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723668) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA723668) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723668) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.