Mouse Interferon alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH14-75]
cat.: HA723669
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Interferon alpha-1 aa 24-189 (HA210987). |
| Positive control: | Recombinant Mouse Interferon alpha 1 protein (HA210987). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-76] to Mouse Interferon alpha 1 antibody (Detector) (HA723670) and Recombinant Mouse Interferon alpha 1 protein (HA210987) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P01572 Mouse |
| Alternative names: | IFL IFN alpha IFN alpha 1/13 IFN alpha 1b IFN alphaD IFN IFN-alpha-1/13 IFNA@ IFNA1 IFNA1_HUMAN IFNA13 Interferon alpha 1 Interferon alpha-1/13 Interferon alpha-D Interferon alpha1 Interferon, alpha 13 LeIF D |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723670, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of spiked IFN-alpha in mouse cell culture media samples. Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.