Human Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PSH14-79]
cat.: HA723674
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Myeloperoxidase aa 49-745 (HA211022). |
| Positive control: | Recombinant Human Myeloperoxidase protein (HA211022). |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-78] to Human Myeloperoxidase antibody (Capture) (HA723673) and Recombinant Human Myeloperoxidase protein (HA211022) as the standard. The reference range value is 93.8-12,000 pg/mL. |
| Uniprot #: | SwissProt: P05164 Human |
| Alternative names: | 84 kDa myeloperoxidase 89 kDa myeloperoxidase EC 1.11.1.7 EC1.11.2.2 fj80f04 MPO mpx myeloid-specific peroxidase Myeloperoxidase Myeloperoxidase heavy chain Myeloperoxidase light chain PERM_HUMAN wu:fj80f04 |
Images
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Fig1:
Sandwich ELISA analysis of Human Myeloperoxidase matched pair antibodies Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78] Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723673) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Myeloperoxidase protein (HA211022) starting from 12,000 pg/ml to 0 pg/ml and detect antibody (HA723674, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Myeloperoxidase in human samples. Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78] Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79] Interpolated concentration of native Myeloperoxidase was measured in duplicate at different sample concentrations and interpolated from the Myeloperoxidase standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 432 ng/mL in HL-60 cell supernatant. There was no detectable signal in Hela cell supernatant. |
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Fig3:
Interpolated concentrations of spiked Myeloperoxidase in cell culture media samples. Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78] Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79] The concentrations of Myeloperoxidase were measured in duplicates, interpolated from the Myeloperoxidase standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.