HRP Conjugated Human Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PSH14-79]
cat.: HA723675H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH14-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Myeloperoxidase aa 49-745 (HA211022). |
| Positive control: | Recombinant Human Myeloperoxidase protein (HA211022). |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-78] to Human Myeloperoxidase antibody (Capture) (HA723673) and Recombinant Human Myeloperoxidase protein (HA211022) as the standard. The reference range value is 31,250-4,000,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P05164 Human |
| Alternative names: | 84 kDa myeloperoxidase 89 kDa myeloperoxidase EC 1.11.1.7 EC1.11.2.2 fj80f04 MPO mpx myeloid-specific peroxidase Myeloperoxidase Myeloperoxidase heavy chain Myeloperoxidase light chain PERM_HUMAN wu:fj80f04 |
Images
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Fig1:
Sandwich ELISA analysis of Human Myeloperoxidase matched pair antibodies Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78] Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723673) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Myeloperoxidase protein (HA211022) starting from 4,000,000 pg/ml to 0 pg/ml and detect antibody (HA723674, HRP, 2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.