Human RBP4 Recombinant Rabbit Monoclonal Antibody [PSH14-89]
cat.: HA723689
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH14-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RBP4 aa 19-201 (HA210999). |
| Positive control: | Recombinant Human RBP4 protein (HA210999). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-88] to Human RBP4 antibody (Capture) (HA723688) and Recombinant Human RBP4 protein (HA210999) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P02753 Human |
| Alternative names: | OTTHUMP00000020114 OTTHUMP00000020115 OTTHUMP00000020116 Plasma retinol binding protein 4 Plasma retinol-binding protein Plasma retinol-binding protein(1-176) PRBP PRO2222 RBP RBP4 RDCCAS RET4_HUMAN Retinol binding protein 4 Retinol binding protein 4 interstitial Retinol binding protein 4 plasma |
Images
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Fig1:
Sandwich ELISA analysis of Human RBP4 matched pair antibodies Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88] Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723688) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RBP4 protein (HA210999) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723689, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native RBP4 in human samples. Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88] Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89] Interpolated concentration of native RBP4 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 208,320 pg/mL in HepG2 cell supernatant. There was no detectable signal in Hela cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked RBP4 in cell culture media samples. Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88] Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89] The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.