HRP Conjugated Human RBP4 Recombinant Rabbit Monoclonal Antibody [PSH14-89]
cat.: HA723690H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH14-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RBP4 aa 19-201 (HA210999). |
| Positive control: | Recombinant Human RBP4 protein (HA210999). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-88] to Human RBP4 antibody (Capture) (HA723688) and Recombinant Human RBP4 protein (HA210999) as the standard. The reference range value is 1,370-37,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02753 Human |
| Alternative names: | OTTHUMP00000020114 OTTHUMP00000020115 OTTHUMP00000020116 Plasma retinol binding protein 4 Plasma retinol-binding protein Plasma retinol-binding protein(1-176) PRBP PRO2222 RBP RBP4 RDCCAS RET4_HUMAN Retinol binding protein 4 Retinol binding protein 4 interstitial Retinol binding protein 4 plasma |
Images
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Fig1:
Sandwich ELISA analysis of Human RBP4 matched pair antibodies Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88] Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723688) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RBP4 protein (HA210999) starting from 37,000 pg/ml to 0 pg/ml and detect antibody (HA723689, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.