VDAC1 / 2 / 3 Recombinant Rabbit Monoclonal Antibody [PSH14-90]
cat.: HA723692
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH14-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human VDAC2 aa 1-294. |
| Positive control: | MCF7 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Mitochondrion outer membrane, Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P21796 Human | P45880 Human | Q9Y277 Human | Q60932 Mouse | Q60930 Mouse | Q60931 Mouse | Q9Z2L0 Rat | P81155 Rat | Q9R1Z0 Rat |
| Alternative names: | hVDAC1 MGC111064 Mithocondrial porin OPM2, yeast, human complement of Outer mitochondrial membrane protein porin 1 Plasmalemmal porin Porin 31 HL Porin 31 HM Porin 31HL Porin 31HM Porin VDAC 1 VDAC VDAC-1 Vdac1 VDAC1_HUMAN Voltage Dependent Anion Channel 1 voltage dependent anion selective channel protein 1 Voltage-dependent anion-selective channel protein 1 B-36 vdac FLJ23841 hVDAC2 OTTHUMP00000019878 OTTHUMP00000019879 OTTHUMP00000019880 Outer mitochondrial membrane protein porin 2 POR Porin-2 VDAC 2 VDAC-2 VDAC2 VDAC2_HUMAN Vdac6 Voltage dependent anion channel 2 Voltage dependent anion selective channel protein 2 Voltage-dependent anion-selective channel protein 2 Non-selective voltage-gated ion channel VDAC3 VDAC-3; hVDAC3 Outer mitochondrial membrane protein porin 3 VDAC3 |
Images
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Fig1:
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA723692) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723692) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA723692) at 1/5,000 dilution. Lane 1: Human VDAC2 recombinant protein Lane 2: Human VDAC1 recombinant protein Lane 3: Human VDAC3 recombinant protein Lysates/proteins at 30 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723692) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.