Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH14-91]
cat.: HA723693
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Eph receptor B2 aa 1-543. |
| Positive control: | COLO205 cell lysate, Saos-2 cell lysate, SW480 cell lysate, COLO205. |
| Subcellular location: | Cell membrane, Cell projection, axon, , dendrite. |
| Recommended Dilutions:
WB IF-Cell IP |
1:5,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: P29323 Human |
| Alternative names: | cek5 Developmentally regulated EPH related tyrosine kinase DRT EK5 ELK related protein tyrosine kinase Eph receptor B2 EPH tyrosine kinase 3 EPH-like kinase 5 EPHB2 EPHB2_HUMAN Ephrin type B receptor 2 Ephrin type-B receptor 2 EPHT 3 ERK ETECK hEK5 Nuk Prkm 5 Receptor protein tyrosine kinase HEK 5 Renal carcinoma antigen NY-REN-47 Sek 3 Tyro 5 Tyrosine protein kinase receptor CEK 5 Tyrosine protein kinase receptor EPH 3 Tyrosine protein kinase receptor QEK 5 Tyrosine-protein kinase receptor EPH-3 Tyrosine-protein kinase TYRO5 |
Images
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Fig1:
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/5,000 dilution. Lane 1: COLO205 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Saos-2 cell lysate Lane 4: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: Lane 1-3: 20 seconds; Lane 4: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723693) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of COLO205 (positive) and K-562 (negative) labeling Eph receptor B2 with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Eph receptor B2 was immunoprecipitated from 0.2 mg COLO205 cell lysate with HA723693 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723693 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: COLO205 cell lysate (input) Lane 2: HA723693 IP in COLO205 cell lysate Lane 3: Rabbit IgG instead of HA723693 in COLO205 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.