Human EphA2 Recombinant Rabbit Monoclonal Antibody [PSH15-12]
cat.: HA723714
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EPHA2 aa 24-534 (HA211075). |
| Positive control: | Recombinant Human EphA2 protein (HA211075). |
| Subcellular location: | Cell membrane, Cell projection, ruffle membrane, lamellipodium membrane, Cell junction, focal adhesion. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-13] to Human EphA2 antibody (Detector) (HA723715) and Recombinant Human EphA2 protein (HA211075) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P29317 Human |
| Alternative names: | ARCC2 AW545284 CTPA CTPP1 CTRCT6 EC 2.7.10.1 Eck Eph receptor A2 EPHA2 EPHA2_HUMAN Ephrin receptor Ephrin receptor EphA2 Ephrin type A receptor 2 Ephrin type-A receptor 2 Epithelial cell kinase Epithelial cell receptor protein tyrosine kinase Myk 2 Myk2 Sek 2 Sek2 Soluble EPHA2 variant 1 Tyrosine protein kinase receptor ECK Tyrosine-protein kinase receptor ECK Tyrosine-protein kinase receptor MPK-5 Tyrosine-protein kinase receptor SEK-2 |
Images
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Fig1:
Sandwich ELISA analysis of Human EphA2 matched pair antibodies Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723714) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (HA211075) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723715, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract. |
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Fig3:
Interpolated concentrations of spiked EphA2 in human cell culture media samples. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.