Human LAMC2 Recombinant Rabbit Monoclonal Antibody [PSH15-15]
cat.: HA723718
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LAMC2 aa 603-1,193 (HA211219). |
| Positive control: | Recombinant Human LAMC2 protein (HA211219). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-14] to Human LAMC2 antibody (Capture) (HA723717) and Recombinant Human LAMC2 protein (HA211219) as the standard. The reference range value is 23.4-6,000 pg/mL. |
| Uniprot #: | SwissProt: Q13753 Human |
| Alternative names: | 3918 B2T BM600 Cell-scattering factor 140 kDa subunit CSF 140 kDa subunit CSF EBR2 EBR2A Epiligrin subunit gamma Kalinin subunit gamma Kalinin/nicein/epiligrin 100 kDa subunit Ladsin 140 kDa subunit LAMB2T LAMC2 LAMC2_HUMAN Laminin 5 gamma 2 subunit Laminin B2t chain Laminin gamma 2 laminin gamma 2 chain Laminin subunit gamma-2 Laminin-5 subunit gamma LAMNB2 Large adhesive scatter factor 140 kDa subunit MGC138491 MGC141938 Nicein subunit gamma NICEIN-100KDA |
Images
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Fig1:
Sandwich ELISA analysis of human LAMC2 matched pair antibodies Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723717) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human LAMC2 protein (HA211219) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723718, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native LAMC2 in human samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Interpolated concentration of native LAMC2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 105,837 pg/mL in A431 and 1,733 pg/ml in A549 cell culture supernatant. There was no detectable signal in HepG2 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked LAMC2 in cell culture media samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.